產(chǎn)品英文名稱(chēng): EasySep™ Human Memory B Cell Isolation Kit
產(chǎn)品中文名稱(chēng): EasySep™ 人類(lèi)的記憶B細(xì)胞分離試劑盒
EasySep™ Human Memory B Cell Isolation Kit 產(chǎn)品英文描述
The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood nucleated cells. B cells are first pre-enriched by negative selection using EasySep™ Human Memory B Cell Enrichment Cocktail. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes (TAC) recognizing CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, glycophorinA and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Following pre-enrichment, CD27 positive B cells are selected using EasySep™ Human CD27 Positive Selection Cocktail. Cells are targeted with TAC recognizing CD27 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet and remain in the tube while the unwanted cells are poured off. This negative cell fraction can be used to obtain naïve B cells. The selection steps can be fully automated using
EasySep™ 人類(lèi)的記憶B細(xì)胞分離試劑盒推薦使用
Isolation of memory B cells (CD27+) from fresh or previously frozen peripheral blood nucleated cells.
EasySep™ Human Memory B Cell Isolation Kit 產(chǎn)品組成
• EasySep™ Human B Cell Enrichment Cocktail, 2 x 1.0 ml
• EasySep™ Magnetic Particles, 5 x 1.0 ml
• EasySep™ Human CD27 Positive Selection Cocktail, 2 x 1.0 ml
• EasySep™ Magnetic Particles, 1.0 ml
EasySep™ 人類(lèi)的記憶B細(xì)胞分離試劑盒保存方法
EASYSEP® HUMAN MEMORY B CELL ENRICHMENT COCKTAIL
EASYSEP® D MAGNETIC PARTICLES
EASYSEP® HUMAN CD27 POSITIVE SELECTION COCKTAIL
EASYSEP® MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EasySep™ Human Memory B Cell Isolation Kit 系列產(chǎn)品訂購(gòu)信息
Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 19054 EasySep™ Human B Cell Enrichment Kit Immunomagnetic Negative Selection Kit
Stemcell 19254 EasySep™ Human Naive B Cell Enrichment Kit Immunomagnetic Negative Selection Kit
Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
