產品英文名稱：L.lactis HCP ELISA kit
產品中文名稱：L.lactis HCP ELISA ELISA 試劑盒

L.lactis HCP ELISA kit 的應用
該試劑盒已專門開發(fā)和驗證用來檢定在乳酸乳球菌中表達的重組產品中乳酸乳球菌HCPS污染物的存在。該試劑盒利用采自乳酸乳球菌介質HCPs的抗體和親和性純化。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。

L.lactis HCP ELISA 試劑盒的英文描述
This kit has been specifically developed and validated for use in detecting L.lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. The antibodies used in this kit were generated against and affinity purified from HCPs obtained from the spent culture media of a null cell line. Our assay validation has determined that the LOD for this kit is ~0.125ng/mL.

L.lactis HCP ELISA kit 的使用步驟規(guī)則
The L. lactis assay is a two-site immunoenzymetric assay.Samples containing L. lactis HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-L. lactis antibody simultaneously in microtiter strips coated with an affinity purified capture anti-L. lactis antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody.The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of L. lactis HCPs present.

L.lactis HCP ELISA 試劑盒的保存和穩(wěn)定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.

L.lactis HCP ELISA kit 的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5N H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
*本試劑盒只能由合格的技術人員使用。