RANTES是CC趨化因子，可通過(guò)CCR1，CCR3，CCR5和US28（巨細(xì)胞病毒受體）受體傳導(dǎo)信號(hào)。 它是一種趨化單核細(xì)胞，記憶T細(xì)胞（CD4 + / CD45RO），嗜堿性粒細(xì)胞和嗜酸性粒細(xì)胞的趨化劑。RANTES還具有抑制某些HIV-1，HIV-2和猿免疫缺陷病毒（SIV）的能力。
BioGems人類(lèi)RANTES預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒采用固相免疫測(cè)定法，專(zhuān)門(mén)設(shè)計(jì)用于檢測(cè)RANTES，96孔板預(yù)包被RANTES特異性小鼠單克隆抗體。它含有大腸桿菌表達(dá)的重組RANTES。檢測(cè)抗體是RANTES特異性的生物素化山羊多克隆抗體。該試劑盒已經(jīng)過(guò)分析驗(yàn)證，是即用型試劑。
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<3pg/ml
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Range:
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15.6pg/ml-1000pg/ml
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Specificity:
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Natural and recombinant Human Rantes
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Uniprot:
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D0EI67
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components
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Description
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Quantity
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Volume
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Anti-Human Rantes Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human Rantes Standard
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2
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10 ng vial
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Human Rantes Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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147
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293
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501
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110
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339
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658
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Standard Deviation
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5.88
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15.53
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31.1
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8.14
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24.41
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56.6
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Consistency (%CV)
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4
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5.3
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6.2
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7.4
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7.2
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8.6
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Description
RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).
The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.
