產(chǎn)品編號：HY-001455

BioGems 人MIP-3β (CCL19) 預(yù)包被ELISA試劑盒，Human MIP-3β (CCL19) Pre-Coated ELISA Kit

Human MIP-3β (CCL19) Pre-Coated ELISA Kit

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生產(chǎn)廠家： BioGems
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規(guī)格型號： 1 Kit
產(chǎn)品編號： HY-001455
產(chǎn)品種類： 細(xì)胞活力檢測

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MIP-3β是一種CC趨化因子，在胸腺，淋巴結(jié)和活化的骨髓基質(zhì)細(xì)胞中表達(dá)，通過CCR7受體傳導(dǎo)。 MIP-3β是T和B淋巴細(xì)胞和骨髓祖細(xì)胞的趨化因子。

BioGems人MIP-3β（CCL19）預(yù)包被ELISA（酶聯(lián)免疫吸附測定）試劑盒采用固相免疫法，專門設(shè)計用于檢測MIP-3β（CCL19），96孔微板預(yù)包被MIP-3β特異鼠單克隆抗體（CCL19）。它含有大腸桿菌表達(dá)的重組MIP-3β（CCL19）。檢測抗體是MIP-3β特異生物素化山羊多克隆抗體（CCL19）。該試劑盒是即用型試劑盒，已經(jīng)過分析驗證。

Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析

Product Details

Assay Type:	Solid Phase Sandwich ELISA: 96-Well Strip Plate
Sensitivity:	<10pg/ml
Range:	15.6pg/ml-1000pg/ml
Specificity:	Natural and recombinant Human MIP-3β?(CCL19)
Uniprot:	Q6IBD6
Interference:	No detectable cross-reactivity with other relevant proteins
Storage Conditions:	Store the unopened product at 2 – 8 ℃.

Components組份

Description	Quantity	Volume
Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate	1	12 strips of 8 wells
Human MIP-3β?(CCL19) Standard	2	10 ng vial
Human MIP-3β?(CCL19) Biotinylated antibody (100x)	1	130 μL
Avidin-Biotin-Peroxidase Complex (100x)	1	130 μL
Sample Diluent	1	30mL
Antibody Diluent	1	12mL
Avidin-Biotin-Peroxidase Diluent	1	12mL
Wash Buffer Concentrate (Powder for 1000mL)	1	Pack
Color Developing Reagent	1	10mL
Stop Solution	1	10mL
Plate Sealers	4	Each

Reproducibility?可重復(fù)性

?	Intra-Assay			Inter-Assay
Sample ID	1	2	3	1	2	3
n=	16	16	16	24	24	24
Mean Measured Concentration (pg/mL)	97	293	583	117	374	627
Standard Deviation	5.53	14.1	36.15	7.5	18.7	42.64
Consistency (%CV)	5.7	4.8	6.2	6.4	5	6.8

Catalog Number : BGK6IBD6

Description

MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β?is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.

The BioGems Human MIP-3β?(CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β?(CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β?(CCL19). It contains E. coli-expressed recombinant MIP-3β?(CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β?(CCL19). The kit is analytically validated with ready to use reagents.

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