產(chǎn)品英文名稱：LEAF™ Purified anti-human CD3
產(chǎn)品中文名稱：LEAF™純化的抗人CD3抗體

BioLegend LEAF™ CD3抗體 純化，抗人英文描述：
Clone:  UCHT1
Isotype:  Mouse IgG1, κ
Isotype Control: LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
Workshop
Number:  III 471
Reactivity:  Human, Cross-Reactivity: Chimpanzee
Formulation:  0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation:  The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration:  1.0 mg/ml
Storage & Handling:  The CD3 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application:  
FC - Quality tested
CyTOF® - Validated
IP, Activ, IHC, WB - Reported in the literature

BioLegend LEAF™ CD3抗體 純化，抗人推薦用法：
每一批這種抗體是免疫熒光染色與流式細(xì)胞儀檢測的質(zhì)量控制。流式細(xì)胞儀染色，建議使用這種試劑是≤2.0每百萬微克細(xì)胞在100μl體積或100微升全血。因此建議該試劑滴定為每個(gè)應(yīng)用程序的最佳性能。

BioLegend LEAF™ CD3抗體 純化，抗人應(yīng)用注意事項(xiàng)：
附加的報(bào)告應(yīng)用程序（有關(guān)格式）包括：丙酮固定的冰凍sections4,6,7和福爾馬林固定的石蠟包埋的sections11，immunoprecipitation1，活化了T cells2,3,5，和西blotting9免疫組織化學(xué)染色。葉™純化抗體（內(nèi)毒素<0.1歐/微克，疊氮化物，無0.2微米過濾）被推薦用于功能分析（目錄300414號）。對于高度敏感的分析，我們建議超LEAF™純化抗體（貨號300438），具有較低的內(nèi)毒素限值比標(biāo)準(zhǔn)LEAF™純化抗體（內(nèi)毒素<0.01 EU /微克）。

BioLegend LEAF™ CD3抗體 純化，抗人實(shí)驗(yàn)圖像：

Human peripheral blood lymphocytes stained with LEAF™ purified UCHT1 and then detected with anti-mouse IgGs FITC

Human peripheral blood mononuclear cells were stained with CFSE on day 0, and then stimulated with (filled histogram) or without (open histogram) immobilized LEAF™ Purified CD3 (clone UCHT1) and LEAF™ purified CD28 (clone CD28.2) for 3 days. On day 4, cells were harvested and stained with CD4 Brilliant Violet 711™. Dot plot (above) was analyzed on live cells. Histogram data (below) was analyzed by gating on CD4 positive cells (above).

 

BioLegend LEAF™ CD3抗體 純化，抗人相關(guān)產(chǎn)品訂購信息：
300413  50 µg   
300414  500 µg   
300432  1 mg