英文名稱(chēng)：Recombinant Mouse Interleukin-4
中文簡(jiǎn)稱(chēng)：【mIL 4】重組小鼠白介素-4
Recombinant Mouse Interleukin-4 【mIL 4】重組小鼠白介素-4 細(xì)胞因子產(chǎn)品描述：
Interleukin-4是主要由產(chǎn)生的多效細(xì)胞激活T淋巴細(xì)胞,嗜堿性粒細(xì)胞和肥大細(xì)胞。多種免疫response-modulating函數(shù)是由IL-4各種細(xì)胞類(lèi)型的調(diào)節(jié)器,它有一個(gè)重要的角色同形像切換、誘導(dǎo)B淋巴細(xì)胞和分化的IgE輔助T細(xì)胞。IL-4綁定由細(xì)胞膜和分泌可溶性IL-4受體。
小鼠白介素-4重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有120個(gè)氨基酸，分子量為13500Da。該小鼠白介素-4重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Mouse Interleukin-4 【mIL 4】重組小鼠白介素-4 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的小鼠白介素-4(mIL 4)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml小鼠白介素-4(mIL 4)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：小鼠白介素-4(mIL 4)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Mouse Interleukin-4 【mIL 4】重組小鼠白介素-4 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Introduction
Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors.
Description
Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton.
The IL-4 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm concentrated (1mg/ml) solution in PBS pH 7.4.
Solubility （溶解性）
It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 10mM HAc not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S.
Biological Activity （生物活性）
The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg.
